Yeast mitochondrial transcription factor Mtf1 determines the precision of promoter-directed initiation of RNA polymerase Rpo41

Xu Yang, Hae Ryung Chang, Yuhui Yin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Despite their clear T7-bacteriophage origin, mitochondrial RNA polymerases have evolved to require transcription factors. All mitochondrial polymerases contain an extra N-terminal domain that has no counterpart in the self-proficient phage enzyme, which is therefore hypothesized to interact with transcription factors. We studied a series of N-terminal deletion mutants of yeast mitochondrial RNA polymerase, Rpo41, and have found that the N-terminal region does not abolish the effects of Mtf1; rather it contributes directly to enzyme catalysis. Mtf1 can rescue the defective Rpo41 enzymes resulted from N-terminal domain deletions. Although Rpo41 appears to have retained all promoter recognition elements found in T7 RNAP, the elements are not independently functional, and Mtf1 is necessary and sufficient for holoenzyme promoter-directed transcription activity.

Original languageEnglish (US)
Article numbere0136879
JournalPLoS One
Volume10
Issue number9
DOIs
StatePublished - Sep 2 2015

Fingerprint

DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
Yeast
Bacteriophages
Transcription Factors
transcription factors
Yeasts
promoter regions
yeasts
bacteriophages
Enzymes
enzymes
Bacteriophage T7
Holoenzymes
Transcription
Catalysis
catalytic activity
transcription (genetics)
mutants
mitochondrial RNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Yeast mitochondrial transcription factor Mtf1 determines the precision of promoter-directed initiation of RNA polymerase Rpo41. / Yang, Xu; Chang, Hae Ryung; Yin, Yuhui.

In: PLoS One, Vol. 10, No. 9, e0136879, 02.09.2015.

Research output: Contribution to journalArticle

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