Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells

T. N. Ledger; B. K. Sil; L. M. Dunster; J. R. Stephenson; P. D. Minor; A. D.T. Barrett

doi:10.1016/0264-410X(92)90084-W

Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells

T. N. Ledger, B. K. Sil, L. M. Dunster, J. R. Stephenson, P. D. Minor, A. D.T. Barrett

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5°C versus 37.0°C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.

Original language	English (US)
Pages (from-to)	652-654
Number of pages	3
Journal	Vaccine
Volume	10
Issue number	10
DOIs	https://doi.org/10.1016/0264-410X(92)90084-W
State	Published - 1992
Externally published	Yes

Keywords

Yellow fever virus vaccines
temperature sensitivity

ASJC Scopus subject areas

Molecular Medicine
General Immunology and Microbiology
General Veterinary
Public Health, Environmental and Occupational Health
Infectious Diseases

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10.1016/0264-410X(92)90084-W

Cite this

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title = "Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells",

abstract = "Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5°C versus 37.0°C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.",

keywords = "Yellow fever virus vaccines, temperature sensitivity",

author = "Ledger, {T. N.} and Sil, {B. K.} and Dunster, {L. M.} and Stephenson, {J. R.} and Minor, {P. D.} and Barrett, {A. D.T.}",

year = "1992",

doi = "10.1016/0264-410X(92)90084-W",

language = "English (US)",

volume = "10",

pages = "652--654",

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TY - JOUR

T1 - Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells

AU - Ledger, T. N.

AU - Sil, B. K.

AU - Dunster, L. M.

AU - Stephenson, J. R.

AU - Minor, P. D.

AU - Barrett, A. D.T.

PY - 1992

Y1 - 1992

N2 - Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5°C versus 37.0°C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.

AB - Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5°C versus 37.0°C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.

KW - Yellow fever virus vaccines

KW - temperature sensitivity

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Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells

Abstract

Keywords

ASJC Scopus subject areas

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