Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells

T. N. Ledger, B. K. Sil, L. M. Dunster, J. R. Stephenson, P. D. Minor, Alan Barrett

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5°C versus 37.0°C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.

Original languageEnglish (US)
Pages (from-to)652-654
Number of pages3
JournalVaccine
Volume10
Issue number10
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Yellow Fever Vaccine
Yellow fever virus
Culicidae
vaccines
Viruses
viruses
Temperature
temperature
cells
Aedes albopictus
Aedes
adenocarcinoma
Brazil
Adenocarcinoma
Vaccines
cell lines
Cell Line

Keywords

  • temperature sensitivity
  • Yellow fever virus vaccines

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells. / Ledger, T. N.; Sil, B. K.; Dunster, L. M.; Stephenson, J. R.; Minor, P. D.; Barrett, Alan.

In: Vaccine, Vol. 10, No. 10, 1992, p. 652-654.

Research output: Contribution to journalArticle

Ledger, T. N. ; Sil, B. K. ; Dunster, L. M. ; Stephenson, J. R. ; Minor, P. D. ; Barrett, Alan. / Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells. In: Vaccine. 1992 ; Vol. 10, No. 10. pp. 652-654.
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AU - Barrett, Alan

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