Yellow fever 17DD vaccine virus is temperature sensitive when grown in mosquito C6-36 cells

T. N. Ledger, B. K. Sil, L. M. Dunster, J. R. Stephenson, P. D. Minor, A. D.T. Barrett

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Twenty-three yellow fever (YF) vaccine viruses and three wild-type YF viruses were propagated independently in human adenocarcinoma (SW13) cells and mosquito Aedes albopictus C6-36 cells. The three YF 17DD vaccine viruses were found to be slightly temperature sensitive (ts) at 39.5°C versus 37.0°C (efficiency of plaquing 0.04 to 0.1) following propagation in C6-36 but not in SW13 cells. A plaque-purified preparation of the 17DD vaccine manufactured in Brazil was ts when grown in C6-36 cells and remained ts when passaged back into the SW13 cell line.

Original languageEnglish (US)
Pages (from-to)652-654
Number of pages3
JournalVaccine
Volume10
Issue number10
DOIs
StatePublished - 1992
Externally publishedYes

Keywords

  • Yellow fever virus vaccines
  • temperature sensitivity

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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