Yellow fever virus envelope protein has two discrete type-specific neutralizing epitopes

K. D. Ryman, T. N. Ledger, R. C. Weir, J. J. Schlesinger, Alan Barrett

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Two monoclonal antibody neutralization resistant (MAb(R)) variants of the yellow fever (YF) 17D-204 vaccine virus strain were selected using YF type-specific MAb B39. These B39(R) variants were compared with the variant virus selected by Lobigs et al. using a second YF-type specific MAb (2E10) which mapped to amino acid position 71/72 in the envelope (E) protein. Neutralization assays with a panel of MAbs suggested that these two YF-type-specific epitopes are located in two discrete regions of the folded E protein. Each of the B39(R) variants had a single nucleotide mutation which encoded an amino acid substitution at either position E-155 or E-158. Thus, YF type-specific epitopes map to both domain I (B39) and II (2E10) of the YF virus E protein. The B39 defined epitope represents the first flavivirus neutralizing epitope localized to this region of domain I of the E protein.

Original languageEnglish (US)
Pages (from-to)1353-1356
Number of pages4
JournalJournal of General Virology
Volume78
Issue number6
StatePublished - Jun 1997

    Fingerprint

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Ryman, K. D., Ledger, T. N., Weir, R. C., Schlesinger, J. J., & Barrett, A. (1997). Yellow fever virus envelope protein has two discrete type-specific neutralizing epitopes. Journal of General Virology, 78(6), 1353-1356.