ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase

Eric Wagner, William F. Marzluff

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Metazoan replication-dependent histone mRNAs are the only eukaryotic mRNAs that are not polyadenylated. The cleavage of histone pre-mRNA to form the unique 3′ end requires the U7 snRNP and the stem-loop binding protein (SLBP) that binds the 3′ end of histone mRNA. 117 snRNP contains three novel proteins, Lsm10 and Lsm11, which are part of the core U7 Sm complex, and ZFP100, a Zn finger protein that helps stabilize binding of the U7 snRNP to the histone pre-mRNA by interacting with the SLBP/pre-mRNA complex. Using a reporter gene that encodes a green fluorescent protein mRNA ending in a histone 3′ end and mimics histone gene expression, we demonstrate that ZFP100 is the limiting factor for histone pre-mRNA processing in vivo. The overexpression of Lsm10 and Lsm11 increases the cellular levels of U7 snRNP but has no effect on histone pre-mRNA processing, while increasing the amount of ZFP100 increases histone pre-mRNA processing but has no effect on U7 snRNP levels. We also show that knocking down the known components of 117 snRNP by RNA interference results in a reduction in cell growth and an unsuspected cell cycle arrest in early G 1, suggesting that active 117 snRNP is necessary to allow progression through G1 phase to S phase.

Original languageEnglish (US)
Pages (from-to)6702-6712
Number of pages11
JournalMolecular and Cellular Biology
Volume26
Issue number17
DOIs
StatePublished - Sep 2006
Externally publishedYes

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U7 Small Nuclear Ribonucleoproteins
RNA Precursors
S Phase
Histones
Small Nuclear Ribonucleoproteins
Messenger RNA
Carrier Proteins
G1 Phase
RNA Interference
Cell Cycle Checkpoints
Green Fluorescent Proteins
Reporter Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

ZFP100, a component of the active U7 snRNP limiting for histone pre-mRNA processing, is required for entry into S phase. / Wagner, Eric; Marzluff, William F.

In: Molecular and Cellular Biology, Vol. 26, No. 17, 09.2006, p. 6702-6712.

Research output: Contribution to journalArticle

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