Zika virus alters the microRNA expression profile and elicits an RNAi response in Aedes aegypti mosquitoes

Miguel A. Saldaña, Kayvan Etebari, Charles E. Hart, Steven Widen, Thomas Wood, Saravanan Thangamani, Sassan Asgari, Grant L. Hughes

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Zika virus (ZIKV), a flavivirus transmitted primarily by Aedes aegypti, has recently spread globally in an unprecedented fashion, yet we have a poor understanding of host-microbe interactions in this system. To gain insights into the interplay between ZIKV and the mosquito, we sequenced the small RNA profiles in ZIKV-infected and non-infected Ae. aegypti mosquitoes at 2, 7 and 14 days post-infection. ZIKA induced an RNAi response in the mosquito with virus-derived short interfering RNAs and PIWI-interacting RNAs dramatically increased in abundance post-infection. Further, we found 17 host microRNAs (miRNAs) that were modulated by ZIKV infection at all time points. Strikingly, many of these regulated miRNAs have been reported to have their expression altered by dengue and West Nile viruses, while the response was divergent from that induced by the alphavirus Chikungunya virus in mosquitoes. This suggests that conserved miRNA responses occur within mosquitoes in response to flavivirus infection. This study expands our understanding of ZIKV-vector interactions and provides potential avenues to be further investigated to target ZIKV in the mosquito host.

Original languageEnglish (US)
Article numbere0005760
JournalPLoS Neglected Tropical Diseases
Volume11
Issue number7
DOIs
StatePublished - Jul 17 2017

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Aedes
RNA Interference
Culicidae
MicroRNAs
Flavivirus Infections
Chikungunya virus
RNA
Alphavirus
Flavivirus
West Nile virus
Dengue
Infection
Small Interfering RNA
Zika Virus
Viruses

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Zika virus alters the microRNA expression profile and elicits an RNAi response in Aedes aegypti mosquitoes. / Saldaña, Miguel A.; Etebari, Kayvan; Hart, Charles E.; Widen, Steven; Wood, Thomas; Thangamani, Saravanan; Asgari, Sassan; Hughes, Grant L.

In: PLoS Neglected Tropical Diseases, Vol. 11, No. 7, e0005760, 17.07.2017.

Research output: Contribution to journalArticle

Saldaña, Miguel A. ; Etebari, Kayvan ; Hart, Charles E. ; Widen, Steven ; Wood, Thomas ; Thangamani, Saravanan ; Asgari, Sassan ; Hughes, Grant L. / Zika virus alters the microRNA expression profile and elicits an RNAi response in Aedes aegypti mosquitoes. In: PLoS Neglected Tropical Diseases. 2017 ; Vol. 11, No. 7.
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