Zika virus in the Americas: Early epidemiological and genetic findings

Nuno Rodrigues Faria, Raimunda Do Socorro Da Silva Azevedo, Moritz U G Kraemer, Renato Souza, Mariana Sequetin Cunha, Sarah C. Hill, Julien Thézé, Michael B. Bonsall, Thomas A. Bowden, Ilona Rissanen, Iray Maria Rocco, Juliana Silva Nogueira, Adriana Yurika Maeda, Fernanda Giseli Da Silva Vasami, Fernando Luiz De Lima Macedo, Akemi Suzuki, Sueli Guerreiro Rodrigues, Ana Cecilia Ribeiro Cruz, Bruno Tardeli Nunes, Daniele Barbosa De Almeida MedeirosDaniela Sueli Guerreiro Rodrigues, Alice Louize Nunes Queiroz, Eliana Vieira Pinto Da Silva, Daniele Freitas Henriques, Elisabeth Salbe Travassos Da Rosa, Consuelo Silva De Oliveira, Livia Caricio Martins, Helena Baldez Vasconcelos, Livia Medeiros Neves Casseb, Darlene De Brito Simith, Jane P. Messina, Leandro Abade, José Lourenço, Luiz Carlos Alcantara, Maricélia Maia De Lima, Marta Giovanetti, Simon I. Hay, Rodrigo Santos De Oliveira, Poliana Da Silva Lemos, Layanna Freitas De Oliveira, Clayton Pereira Silva De Lima, Sandro Patroca Da Silva, Janaina Mota De Vasconcelos, Luciano Franco, Jedson Ferreira Cardoso, João Lídio Da Silva Gonçalves Vianez-Júnior, Daiana Mir, Gonzalo Bello, Edson Delatorre, Kamran Khan, Marisa Creatore, Giovanini Evelim Coelho, Wanderson Kleber De Oliveira, Robert Tesh, Oliver G. Pybus, Marcio R T Nunes, Pedro F C Vasconcelos

Research output: Contribution to journalArticle

491 Citations (Scopus)

Abstract

Brazil has experienced an unprecedented epidemic of Zika virus (ZIKV), with ∼30,000 cases reported to date. ZIKV was first detected in Brazil in May 2015, and cases of microcephaly potentially associated with ZIKV infection were identified in November 2015. We performed next-generation sequencing to generate seven Brazilian ZIKV genomes sampled from four self-limited cases, one blood donor, one fatal adult case, and one newborn with microcephaly and congenital malformations. Results of phylogenetic and molecular clock analyses show a single introduction of ZIKV into the Americas, which we estimated to have occurred between May and December 2013, more than 12 months before the detection of ZIKV in Brazil. The estimated date of origin coincides with an increase in air passengers to Brazil from ZIKV-endemic areas, as well as with reported outbreaks in the Pacific Islands. ZIKV genomes from Brazil are phylogenetically interspersed with those from other South American and Caribbean countries. Mapping mutations onto existing structural models revealed the context of viral amino acid changes present in the outbreak lineage; however, no shared amino acid changes were found among the three currently available virus genomes from microcephaly cases. Municipality-level incidence data indicate that reports of suspected microcephaly in Brazil best correlate with ZIKV incidence around week 17 of pregnancy, although this correlation does not demonstrate causation. Our genetic description and analysis of ZIKV isolates in Brazil provide a baseline for future studies of the evolution and molecular epidemiology of this emerging virus in the Americas.

Original languageEnglish (US)
Pages (from-to)345-349
Number of pages5
JournalScience
Volume352
Issue number6283
DOIs
StatePublished - Apr 15 2016

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Brazil
Microcephaly
Genome
Disease Outbreaks
Pacific Islands
Viruses
Zika Virus
Amino Acids
Molecular Epidemiology
Structural Models
Incidence
Blood Donors
Causality
Air
Newborn Infant
Pregnancy
Mutation

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Faria, N. R., Do Socorro Da Silva Azevedo, R., Kraemer, M. U. G., Souza, R., Cunha, M. S., Hill, S. C., ... Vasconcelos, P. F. C. (2016). Zika virus in the Americas: Early epidemiological and genetic findings. Science, 352(6283), 345-349. https://doi.org/10.1126/science.aaf5036

Zika virus in the Americas : Early epidemiological and genetic findings. / Faria, Nuno Rodrigues; Do Socorro Da Silva Azevedo, Raimunda; Kraemer, Moritz U G; Souza, Renato; Cunha, Mariana Sequetin; Hill, Sarah C.; Thézé, Julien; Bonsall, Michael B.; Bowden, Thomas A.; Rissanen, Ilona; Rocco, Iray Maria; Nogueira, Juliana Silva; Maeda, Adriana Yurika; Da Silva Vasami, Fernanda Giseli; De Lima Macedo, Fernando Luiz; Suzuki, Akemi; Rodrigues, Sueli Guerreiro; Cruz, Ana Cecilia Ribeiro; Nunes, Bruno Tardeli; De Almeida Medeiros, Daniele Barbosa; Rodrigues, Daniela Sueli Guerreiro; Queiroz, Alice Louize Nunes; Da Silva, Eliana Vieira Pinto; Henriques, Daniele Freitas; Da Rosa, Elisabeth Salbe Travassos; De Oliveira, Consuelo Silva; Martins, Livia Caricio; Vasconcelos, Helena Baldez; Casseb, Livia Medeiros Neves; De Brito Simith, Darlene; Messina, Jane P.; Abade, Leandro; Lourenço, José; Alcantara, Luiz Carlos; De Lima, Maricélia Maia; Giovanetti, Marta; Hay, Simon I.; De Oliveira, Rodrigo Santos; Da Silva Lemos, Poliana; De Oliveira, Layanna Freitas; De Lima, Clayton Pereira Silva; Da Silva, Sandro Patroca; De Vasconcelos, Janaina Mota; Franco, Luciano; Cardoso, Jedson Ferreira; Da Silva Gonçalves Vianez-Júnior, João Lídio; Mir, Daiana; Bello, Gonzalo; Delatorre, Edson; Khan, Kamran; Creatore, Marisa; Coelho, Giovanini Evelim; De Oliveira, Wanderson Kleber; Tesh, Robert; Pybus, Oliver G.; Nunes, Marcio R T; Vasconcelos, Pedro F C.

In: Science, Vol. 352, No. 6283, 15.04.2016, p. 345-349.

Research output: Contribution to journalArticle

Faria, NR, Do Socorro Da Silva Azevedo, R, Kraemer, MUG, Souza, R, Cunha, MS, Hill, SC, Thézé, J, Bonsall, MB, Bowden, TA, Rissanen, I, Rocco, IM, Nogueira, JS, Maeda, AY, Da Silva Vasami, FG, De Lima Macedo, FL, Suzuki, A, Rodrigues, SG, Cruz, ACR, Nunes, BT, De Almeida Medeiros, DB, Rodrigues, DSG, Queiroz, ALN, Da Silva, EVP, Henriques, DF, Da Rosa, EST, De Oliveira, CS, Martins, LC, Vasconcelos, HB, Casseb, LMN, De Brito Simith, D, Messina, JP, Abade, L, Lourenço, J, Alcantara, LC, De Lima, MM, Giovanetti, M, Hay, SI, De Oliveira, RS, Da Silva Lemos, P, De Oliveira, LF, De Lima, CPS, Da Silva, SP, De Vasconcelos, JM, Franco, L, Cardoso, JF, Da Silva Gonçalves Vianez-Júnior, JL, Mir, D, Bello, G, Delatorre, E, Khan, K, Creatore, M, Coelho, GE, De Oliveira, WK, Tesh, R, Pybus, OG, Nunes, MRT & Vasconcelos, PFC 2016, 'Zika virus in the Americas: Early epidemiological and genetic findings', Science, vol. 352, no. 6283, pp. 345-349. https://doi.org/10.1126/science.aaf5036
Faria NR, Do Socorro Da Silva Azevedo R, Kraemer MUG, Souza R, Cunha MS, Hill SC et al. Zika virus in the Americas: Early epidemiological and genetic findings. Science. 2016 Apr 15;352(6283):345-349. https://doi.org/10.1126/science.aaf5036
Faria, Nuno Rodrigues ; Do Socorro Da Silva Azevedo, Raimunda ; Kraemer, Moritz U G ; Souza, Renato ; Cunha, Mariana Sequetin ; Hill, Sarah C. ; Thézé, Julien ; Bonsall, Michael B. ; Bowden, Thomas A. ; Rissanen, Ilona ; Rocco, Iray Maria ; Nogueira, Juliana Silva ; Maeda, Adriana Yurika ; Da Silva Vasami, Fernanda Giseli ; De Lima Macedo, Fernando Luiz ; Suzuki, Akemi ; Rodrigues, Sueli Guerreiro ; Cruz, Ana Cecilia Ribeiro ; Nunes, Bruno Tardeli ; De Almeida Medeiros, Daniele Barbosa ; Rodrigues, Daniela Sueli Guerreiro ; Queiroz, Alice Louize Nunes ; Da Silva, Eliana Vieira Pinto ; Henriques, Daniele Freitas ; Da Rosa, Elisabeth Salbe Travassos ; De Oliveira, Consuelo Silva ; Martins, Livia Caricio ; Vasconcelos, Helena Baldez ; Casseb, Livia Medeiros Neves ; De Brito Simith, Darlene ; Messina, Jane P. ; Abade, Leandro ; Lourenço, José ; Alcantara, Luiz Carlos ; De Lima, Maricélia Maia ; Giovanetti, Marta ; Hay, Simon I. ; De Oliveira, Rodrigo Santos ; Da Silva Lemos, Poliana ; De Oliveira, Layanna Freitas ; De Lima, Clayton Pereira Silva ; Da Silva, Sandro Patroca ; De Vasconcelos, Janaina Mota ; Franco, Luciano ; Cardoso, Jedson Ferreira ; Da Silva Gonçalves Vianez-Júnior, João Lídio ; Mir, Daiana ; Bello, Gonzalo ; Delatorre, Edson ; Khan, Kamran ; Creatore, Marisa ; Coelho, Giovanini Evelim ; De Oliveira, Wanderson Kleber ; Tesh, Robert ; Pybus, Oliver G. ; Nunes, Marcio R T ; Vasconcelos, Pedro F C. / Zika virus in the Americas : Early epidemiological and genetic findings. In: Science. 2016 ; Vol. 352, No. 6283. pp. 345-349.
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abstract = "Brazil has experienced an unprecedented epidemic of Zika virus (ZIKV), with ∼30,000 cases reported to date. ZIKV was first detected in Brazil in May 2015, and cases of microcephaly potentially associated with ZIKV infection were identified in November 2015. We performed next-generation sequencing to generate seven Brazilian ZIKV genomes sampled from four self-limited cases, one blood donor, one fatal adult case, and one newborn with microcephaly and congenital malformations. Results of phylogenetic and molecular clock analyses show a single introduction of ZIKV into the Americas, which we estimated to have occurred between May and December 2013, more than 12 months before the detection of ZIKV in Brazil. The estimated date of origin coincides with an increase in air passengers to Brazil from ZIKV-endemic areas, as well as with reported outbreaks in the Pacific Islands. ZIKV genomes from Brazil are phylogenetically interspersed with those from other South American and Caribbean countries. Mapping mutations onto existing structural models revealed the context of viral amino acid changes present in the outbreak lineage; however, no shared amino acid changes were found among the three currently available virus genomes from microcephaly cases. Municipality-level incidence data indicate that reports of suspected microcephaly in Brazil best correlate with ZIKV incidence around week 17 of pregnancy, although this correlation does not demonstrate causation. Our genetic description and analysis of ZIKV isolates in Brazil provide a baseline for future studies of the evolution and molecular epidemiology of this emerging virus in the Americas.",
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T1 - Zika virus in the Americas

T2 - Early epidemiological and genetic findings

AU - Faria, Nuno Rodrigues

AU - Do Socorro Da Silva Azevedo, Raimunda

AU - Kraemer, Moritz U G

AU - Souza, Renato

AU - Cunha, Mariana Sequetin

AU - Hill, Sarah C.

AU - Thézé, Julien

AU - Bonsall, Michael B.

AU - Bowden, Thomas A.

AU - Rissanen, Ilona

AU - Rocco, Iray Maria

AU - Nogueira, Juliana Silva

AU - Maeda, Adriana Yurika

AU - Da Silva Vasami, Fernanda Giseli

AU - De Lima Macedo, Fernando Luiz

AU - Suzuki, Akemi

AU - Rodrigues, Sueli Guerreiro

AU - Cruz, Ana Cecilia Ribeiro

AU - Nunes, Bruno Tardeli

AU - De Almeida Medeiros, Daniele Barbosa

AU - Rodrigues, Daniela Sueli Guerreiro

AU - Queiroz, Alice Louize Nunes

AU - Da Silva, Eliana Vieira Pinto

AU - Henriques, Daniele Freitas

AU - Da Rosa, Elisabeth Salbe Travassos

AU - De Oliveira, Consuelo Silva

AU - Martins, Livia Caricio

AU - Vasconcelos, Helena Baldez

AU - Casseb, Livia Medeiros Neves

AU - De Brito Simith, Darlene

AU - Messina, Jane P.

AU - Abade, Leandro

AU - Lourenço, José

AU - Alcantara, Luiz Carlos

AU - De Lima, Maricélia Maia

AU - Giovanetti, Marta

AU - Hay, Simon I.

AU - De Oliveira, Rodrigo Santos

AU - Da Silva Lemos, Poliana

AU - De Oliveira, Layanna Freitas

AU - De Lima, Clayton Pereira Silva

AU - Da Silva, Sandro Patroca

AU - De Vasconcelos, Janaina Mota

AU - Franco, Luciano

AU - Cardoso, Jedson Ferreira

AU - Da Silva Gonçalves Vianez-Júnior, João Lídio

AU - Mir, Daiana

AU - Bello, Gonzalo

AU - Delatorre, Edson

AU - Khan, Kamran

AU - Creatore, Marisa

AU - Coelho, Giovanini Evelim

AU - De Oliveira, Wanderson Kleber

AU - Tesh, Robert

AU - Pybus, Oliver G.

AU - Nunes, Marcio R T

AU - Vasconcelos, Pedro F C

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AB - Brazil has experienced an unprecedented epidemic of Zika virus (ZIKV), with ∼30,000 cases reported to date. ZIKV was first detected in Brazil in May 2015, and cases of microcephaly potentially associated with ZIKV infection were identified in November 2015. We performed next-generation sequencing to generate seven Brazilian ZIKV genomes sampled from four self-limited cases, one blood donor, one fatal adult case, and one newborn with microcephaly and congenital malformations. Results of phylogenetic and molecular clock analyses show a single introduction of ZIKV into the Americas, which we estimated to have occurred between May and December 2013, more than 12 months before the detection of ZIKV in Brazil. The estimated date of origin coincides with an increase in air passengers to Brazil from ZIKV-endemic areas, as well as with reported outbreaks in the Pacific Islands. ZIKV genomes from Brazil are phylogenetically interspersed with those from other South American and Caribbean countries. Mapping mutations onto existing structural models revealed the context of viral amino acid changes present in the outbreak lineage; however, no shared amino acid changes were found among the three currently available virus genomes from microcephaly cases. Municipality-level incidence data indicate that reports of suspected microcephaly in Brazil best correlate with ZIKV incidence around week 17 of pregnancy, although this correlation does not demonstrate causation. Our genetic description and analysis of ZIKV isolates in Brazil provide a baseline for future studies of the evolution and molecular epidemiology of this emerging virus in the Americas.

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