Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease

Benjamin M. Akiyama, Hannah M. Laurence, Aaron R. Massey, David A. Costantino, Xuping Xie, Yujiao Yang, Pei-Yong Shi, Jay C. Nix, J. David Beckham, Jeffrey S. Kieft

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The outbreak of Zika virus (ZIKV) and associated fetal microcephaly mandates efforts to understand the molecular processes of infection. Related flaviviruses produce noncoding subgenomic flaviviral RNAs (sfRNAs) that are linked to pathogenicity in fetal mice. These viruses make sfRNAs by co-opting a cellular exonuclease via structured RNAs called xrRNAs. We found that ZIKV-infected monkey and human epithelial cells, mouse neurons, and mosquito cells produce sfRNAs. The RNA structure that is responsible for ZIKV sfRNA production forms a complex fold that is likely found in many pathogenic flaviviruses. Mutations that disrupt the structure affect exonuclease resistance in vitro and sfRNA formation during infection. The complete ZIKV xrRNA structure clarifies the mechanism of exonuclease resistance and identifies features that may modulate function in diverse flaviviruses.

Original languageEnglish (US)
Pages (from-to)1148-1152
Number of pages5
JournalScience
Volume354
Issue number6316
DOIs
StatePublished - Dec 2 2016

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Exonucleases
Untranslated RNA
Flavivirus
RNA
Viral Structures
Microcephaly
Infection
Culicidae
Haplorhini
Disease Outbreaks
Virulence
Epithelial Cells
Zika Virus
Viruses
Neurons
Mutation

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Akiyama, B. M., Laurence, H. M., Massey, A. R., Costantino, D. A., Xie, X., Yang, Y., ... Kieft, J. S. (2016). Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. Science, 354(6316), 1148-1152. https://doi.org/10.1126/science.aah3963

Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. / Akiyama, Benjamin M.; Laurence, Hannah M.; Massey, Aaron R.; Costantino, David A.; Xie, Xuping; Yang, Yujiao; Shi, Pei-Yong; Nix, Jay C.; Beckham, J. David; Kieft, Jeffrey S.

In: Science, Vol. 354, No. 6316, 02.12.2016, p. 1148-1152.

Research output: Contribution to journalArticle

Akiyama, BM, Laurence, HM, Massey, AR, Costantino, DA, Xie, X, Yang, Y, Shi, P-Y, Nix, JC, Beckham, JD & Kieft, JS 2016, 'Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease', Science, vol. 354, no. 6316, pp. 1148-1152. https://doi.org/10.1126/science.aah3963
Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y et al. Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. Science. 2016 Dec 2;354(6316):1148-1152. https://doi.org/10.1126/science.aah3963
Akiyama, Benjamin M. ; Laurence, Hannah M. ; Massey, Aaron R. ; Costantino, David A. ; Xie, Xuping ; Yang, Yujiao ; Shi, Pei-Yong ; Nix, Jay C. ; Beckham, J. David ; Kieft, Jeffrey S. / Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. In: Science. 2016 ; Vol. 354, No. 6316. pp. 1148-1152.
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