Abstract
The current epidemic of Zika virus (ZIKV) has underscored the urgency to establish experimental systems for studying viral replication and pathogenesis, and countermeasure development. Here we report two ZIKV replicon systems: a luciferase replicon that can differentiate between viral translation and RNA synthesis; and a stable luciferase replicon carrying cell line that can be used to screen and characterize inhibitors of viral replication. The transient replicon was used to evaluate the effect of an NS5 polymerase mutation on viral RNA synthesis and to analyze a known ZIKV inhibitor. The replicon cell line was developed into a 96-well format for antiviral testing. Compare with virus infection-based assay, the replicon cell line allows antiviral screening without using infectious virus. Collectively, the replicon systems have provided critical tools for both basic and translational research.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 156-160 |
| Number of pages | 5 |
| Journal | EBioMedicine |
| Volume | 12 |
| DOIs | |
| State | Published - Oct 1 2016 |
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Keywords
- Drug discovery
- Flavivirus
- Replicon
- Zika
ASJC Scopus subject areas
- Medicine(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Zika Virus Replicons for Drug Discovery. / Xie, Xuping; Zou, Jing; Shan, Chao; Yang, Yujiao; Kum, Dieudonné Buh; Dallmeier, Kai; Neyts, Johan; Shi, Pei-Yong.
In: EBioMedicine, Vol. 12, 01.10.2016, p. 156-160.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Zika Virus Replicons for Drug Discovery
AU - Xie, Xuping
AU - Zou, Jing
AU - Shan, Chao
AU - Yang, Yujiao
AU - Kum, Dieudonné Buh
AU - Dallmeier, Kai
AU - Neyts, Johan
AU - Shi, Pei-Yong
PY - 2016/10/1
Y1 - 2016/10/1
N2 - The current epidemic of Zika virus (ZIKV) has underscored the urgency to establish experimental systems for studying viral replication and pathogenesis, and countermeasure development. Here we report two ZIKV replicon systems: a luciferase replicon that can differentiate between viral translation and RNA synthesis; and a stable luciferase replicon carrying cell line that can be used to screen and characterize inhibitors of viral replication. The transient replicon was used to evaluate the effect of an NS5 polymerase mutation on viral RNA synthesis and to analyze a known ZIKV inhibitor. The replicon cell line was developed into a 96-well format for antiviral testing. Compare with virus infection-based assay, the replicon cell line allows antiviral screening without using infectious virus. Collectively, the replicon systems have provided critical tools for both basic and translational research.
AB - The current epidemic of Zika virus (ZIKV) has underscored the urgency to establish experimental systems for studying viral replication and pathogenesis, and countermeasure development. Here we report two ZIKV replicon systems: a luciferase replicon that can differentiate between viral translation and RNA synthesis; and a stable luciferase replicon carrying cell line that can be used to screen and characterize inhibitors of viral replication. The transient replicon was used to evaluate the effect of an NS5 polymerase mutation on viral RNA synthesis and to analyze a known ZIKV inhibitor. The replicon cell line was developed into a 96-well format for antiviral testing. Compare with virus infection-based assay, the replicon cell line allows antiviral screening without using infectious virus. Collectively, the replicon systems have provided critical tools for both basic and translational research.
KW - Drug discovery
KW - Flavivirus
KW - Replicon
KW - Zika
UR - http://www.scopus.com/inward/record.url?scp=84992646605&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84992646605&partnerID=8YFLogxK
U2 - 10.1016/j.ebiom.2016.09.013
DO - 10.1016/j.ebiom.2016.09.013
M3 - Article
C2 - 27658737
AN - SCOPUS:84992646605
VL - 12
SP - 156
EP - 160
JO - EBioMedicine
JF - EBioMedicine
SN - 2352-3964
ER -