ZIKV Demonstrates Minimal Pathologic Effects and Mosquito Infectivity in Viremic Cynomolgus Macaques

Sasha R. Azar, Shannan Rossi, Sherry Haller, Ruimei Yun, Jing H. Huang, Jessica Plante, Jiehua Zhou, Juan Olano, Christopher M. Roundy, Kathryn A. Hanley, Scott Weaver, Nikos Vasilakis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To evaluate the effects of ZIKV infection on non-human primates (NHPs), as well as to investigate whether these NHPs develop sufficient viremia to infect the major urban vector mosquito, Aedes aegypti, four cynomolgus macaques (Macaca fascicularis) were subcutaneously infected with 5.0 log10 focus-forming units (FFU) of DNA clone-derived ZIKV strain FSS13025 (Asian lineage, Cambodia, 2010). Following infection, the animals were sampled (blood, urine, tears, and saliva), underwent daily health monitoring, and were exposed to Ae. aegypti at specified time points. All four animals developed viremia, which peaked 3⁻4 days post-infection at a maximum value of 6.9 log10 genome copies/mL. No virus was detected in urine, tears, or saliva. Infection by ZIKV caused minimal overt disease: serum biochemistry and CBC values largely fell within the normal ranges, and cytokine elevations were minimal. Strikingly, the minimally colonized population of Ae. aegypti exposed to viremic animals demonstrated a maximum infection rate of 26% during peak viremia, with two of the four macaques failing to infect a single mosquito at any time point. These data indicate that cynomolgus macaques may be an effective model for ZIKV infection of humans and highlights the relative refractoriness of Ae. aegypti for ZIKV infection at the levels of viremia observed.

Original languageEnglish (US)
JournalViruses
Volume10
Issue number11
DOIs
StatePublished - Nov 21 2018

Fingerprint

Macaca
Culicidae
Viremia
Infection
Tears
Saliva
Primates
Urine
Cambodia
Macaca fascicularis
Aedes
Biochemistry
Reference Values
Clone Cells
Genome
Cytokines
Viruses
DNA
Health
Serum

Keywords

  • Aedes aegypti
  • Flaviviridae
  • Macaca fascicularis
  • pathogenesis
  • vector competence
  • Zika virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

ZIKV Demonstrates Minimal Pathologic Effects and Mosquito Infectivity in Viremic Cynomolgus Macaques. / Azar, Sasha R.; Rossi, Shannan; Haller, Sherry; Yun, Ruimei; Huang, Jing H.; Plante, Jessica; Zhou, Jiehua; Olano, Juan; Roundy, Christopher M.; Hanley, Kathryn A.; Weaver, Scott; Vasilakis, Nikos.

In: Viruses, Vol. 10, No. 11, 21.11.2018.

Research output: Contribution to journalArticle

Azar, Sasha R. ; Rossi, Shannan ; Haller, Sherry ; Yun, Ruimei ; Huang, Jing H. ; Plante, Jessica ; Zhou, Jiehua ; Olano, Juan ; Roundy, Christopher M. ; Hanley, Kathryn A. ; Weaver, Scott ; Vasilakis, Nikos. / ZIKV Demonstrates Minimal Pathologic Effects and Mosquito Infectivity in Viremic Cynomolgus Macaques. In: Viruses. 2018 ; Vol. 10, No. 11.
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