A single immunization with a modified vaccinia Ankara vectored vaccine producing Sudan virus-like particles protects from lethal infection

Delphine C. Malherbe; Arban Domi; Mary J. Hauser; Caroline Atyeo; Stephanie Fischinger; Matthew A. Hyde; Julie M. Williams; Galit Alter; Farshad Guirakhoo; Alexander Bukreyev

doi:10.1038/s41541-022-00512-x

A single immunization with a modified vaccinia Ankara vectored vaccine producing Sudan virus-like particles protects from lethal infection

Delphine C. Malherbe
, Arban Domi
, Mary J. Hauser
, Caroline Atyeo
, Stephanie Fischinger
, Matthew A. Hyde
, Julie M. Williams
, Galit Alter
, Farshad Guirakhoo
, Alexander Bukreyev

Pathology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A new vectored vaccine MVA-VLP-SUDV was generated against Sudan ebolavirus (SUDV) combining the advantages of the immunogenicity of a live attenuated vaccine vector (Modified Vaccinia Ankara, MVA) with the authentic conformation of virus-like particles (VLPs). The vaccine expresses minimal components to generate self-assembling VLPs in the vaccinee: the envelope glycoprotein GP and the matrix protein VP40. Guinea pigs vaccinated with one dose of MVA-VLP-SUDV generated SUDV-specific binding and neutralizing antibody responses as well as Fc-mediated protective effects. These responses were boosted by a second vaccine dose. All vaccinated animals which received either one or two vaccine doses were protected from death and disease symptoms following challenge with a lethal dose of SUDV. These data demonstrate single dose protection and potency of the MVA-VLP platform for use in emergency situations to contain outbreaks.

Original language	English (US)
Article number	83
Journal	npj Vaccines
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41541-022-00512-x
State	Published - Dec 2022

ASJC Scopus subject areas

Immunology
Pharmacology
Infectious Diseases
Pharmacology (medical)

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10.1038/s41541-022-00512-x

Cite this

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title = "A single immunization with a modified vaccinia Ankara vectored vaccine producing Sudan virus-like particles protects from lethal infection",

abstract = "A new vectored vaccine MVA-VLP-SUDV was generated against Sudan ebolavirus (SUDV) combining the advantages of the immunogenicity of a live attenuated vaccine vector (Modified Vaccinia Ankara, MVA) with the authentic conformation of virus-like particles (VLPs). The vaccine expresses minimal components to generate self-assembling VLPs in the vaccinee: the envelope glycoprotein GP and the matrix protein VP40. Guinea pigs vaccinated with one dose of MVA-VLP-SUDV generated SUDV-specific binding and neutralizing antibody responses as well as Fc-mediated protective effects. These responses were boosted by a second vaccine dose. All vaccinated animals which received either one or two vaccine doses were protected from death and disease symptoms following challenge with a lethal dose of SUDV. These data demonstrate single dose protection and potency of the MVA-VLP platform for use in emergency situations to contain outbreaks.",

author = "Malherbe, \{Delphine C.\} and Arban Domi and Hauser, \{Mary J.\} and Caroline Atyeo and Stephanie Fischinger and Hyde, \{Matthew A.\} and Williams, \{Julie M.\} and Galit Alter and Farshad Guirakhoo and Alexander Bukreyev",

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doi = "10.1038/s41541-022-00512-x",

language = "English (US)",

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AU - Domi, Arban

AU - Hauser, Mary J.

AU - Atyeo, Caroline

AU - Fischinger, Stephanie

AU - Hyde, Matthew A.

AU - Williams, Julie M.

AU - Alter, Galit

AU - Guirakhoo, Farshad

AU - Bukreyev, Alexander

PY - 2022/12

Y1 - 2022/12

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A single immunization with a modified vaccinia Ankara vectored vaccine producing Sudan virus-like particles protects from lethal infection

Abstract

ASJC Scopus subject areas

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